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Chapter 98 Gene Sequence

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    When Yushi heard it, he teased his father straight away: "No matter how powerful Sun Wukong is in the Heavenly Palace, isn't he still crushed under Wuzhi Mountain in the end? This song is not sung correctly, it is not suitable for the scene."

    When Shi Zhiwen heard it, he said: "You brat, you know how to make me suffer. Why can't you see me humming a little song proudly? Ren Rongrong is the one who plays chess with me, she didn't say anything,  What nonsense are you talking about?"

    Speaking of which, Shi Zhiwen turned his head and said to Rongrong: "Rongrong, you come to judge, do you think he talks too much?"

    Hearing this, Rongrong lowered her head and let out a "chi" laugh: "This havoc in the Heavenly Palace requires an imposing manner. Although I don't specialize in learning Kunqu Opera, I have read a lot of it. At least I know that it is necessary to have both spirit and form."  , that¡¯s called good singing. If you want me to say, Uncle¡¯s singing is pretty good, which makes sense.¡±

    "Listen, you all listen, our Rongrong can still talk." Shi Zhiwen pouted proudly.

    Yu Shi smiled: "You've already said that, Rongrong still doesn't turn to you? Anyway, you are right in this family, and I'll just take care of it and mess around."

    When the voice fell to the ground, everyone laughed together.  Ever since talking about friends with Jiang Rongrong, he almost didn't go out much on weekends when it rained.  He honestly took care of the children at home, or watched Rong Rong play erhu and play chess with his father.  He was also happy to fight aside, handing tea or something.

    Rongrong, who is eccentric, always finds ways to make Shi Zhiwen happy.  After coming and going, Shi Zhiwen especially liked the girl Rong Rong, and always said that she was more caring than his own son.  Every time Yu Shi listened to it, he was secretly happy. In his opinion, he was happier to praise his girlfriend than to praise himself.

    Huai Ru and Wan Qing both said that since they were together with Rong Rong when it was raining, the house was like a sunflower growing in the sun.  The life of the whole family, old and young, seems to be on the right track, and it is much more enjoyable than before.

    ¡ª¡ª¡ª¡ª

    Noroviruses are highly species-specific, so current data show that noroviruses collected from humans generally only spread among humans.  And animals such as mice, if they are infected with norovirus, they only spread among their own kind.

    Therefore, Huai Ru and Wan Qing knew that it is obviously not feasible to study human norovirus in mice, rats, rabbits or other models.

    Norovirus samples obtained from humans cannot even infect human cells in culture, which also brings certain difficulties to research and development.

    Since Professor Albert Kapikian first isolated norovirus from the feces of patients with acute diarrhea in 1968, scientists all over the world have been looking for a suitable way to grow the virus in the laboratory, and some laboratories have even spent decades.  No progress.

    On the other hand, Zhang Xingzhi already had the orf2 gene sequence of the gii-4 type norovirus that is prevalent in China in the previous research that had stagnated, which also provided a basis for their further research on vaccines.

    He extracted viral RNA from gii-4 type noro feces, and after reverse transcription, PCR cloned it into a vector, and finally obtained the correct sequence of gene ii-4, subtype noro orf2 gene sequence.

    Huairu, Wan Qing, and Zhang Xingzhi cooperated with each other and re-analyzed with software. The amino acid size encoded by the orf2 sequence of the three norovirus strains was about 54kd, and the amino acid homology with va387 (gii-4) reached about 93%.  (Remember the site URL: www.hlnovel.com
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